Characterization and validation of point mutation in exon 19 of CACNA2D1 gene in Karan Fries (Bos taurus x Bos indicus) cattle

The objective of this study was to characterize and validate the candidate point mutation in Calcium channel, voltage-dependent, alpha-2/ delta subunit 1 (CACNA2D1) gene in Karan Fries (Bos taurus x Bos indicus) cattle. The CACNA2D1 gene reported as one of the potential candidate gene influencing Somatic cell Score and Mastitis. A PCR product of 249 bp amplifying the exon 19 and partial 18 and 19 intronic region of CACNA2D1 gene was digested with Hae III restriction enzyme to screen the reported point mutation. A monomorphic banding pattern with genotype AA was found in Karan Fries cattle. Sequencing was also carried out to characterize and explore insilico screened mutation in the nucleotide sequence of a particular region. The result indicates highly conserved sequence in Karan Fries cattle. The Phylogenetic tree revealed that Karan Fries cattle were closer to Bos taurus cattle, Bos mutus (Yak), and Bison bison (American buffalo) compared to other species.