Kidney physiopathologic changes in diabetic Golden-Syrian hamsters (Mesocricetus auratus) fed with hypercaloric diet

Diabetic nephropathy is the single major cause of end stage renal failure. The increase of visceral adipose tissue may lead to glomerular hypertrophy and chronic kidney disease. The objective is to determine renal changes in diabetic Golden-Syrian Hamster (Mesocricetus auratus) supplemented with a hypercaloric diet. Methods: One group of animals (n=10) was fed with a standard diet (SD), and the other group (n=10) was fed with a hypercaloric diet (HCD) for 1 month. Afterwards, both groups were treated with three doses of Streptozotocin. Hyperglycemia was determined throughout 73 days. The animal’s weight, blood and kidney tissues were obtained for analysis. Results: Diabetic animals fed with HCD diet manifested hyperglycemia (250-350 mg/dL) with significant weight loss (40g) and important glomerular filtration rate decrement (0.491mL/min). Regarding to renal fibrosis, all animals showed an increase of glomerular, interstitial, and cortical extracellular matrix (36.3, 75.2 and 70.7%, respectively). Diabetic animals that were SD-fed showed only mild hyperglycemia and slight increase of glomerular, interstitial, and cortical extracellular matrix. A group of animals (n=5) that was fed exclusively with HCD was also included in the study. Conclusions: Our finding suggests that HCD fed can accelerate the progression of chronic kidney disease in a diabetic condition. La nefropatía diabética es la principal causa de insuficiencia renal terminal. El aumento del tejido adiposo visceral puede provocar hipertrofia glomerular y enfermedad renal crónica. El objetivo es determinar los cambios renales en hámster sirio dorado (Mesocricetus auratus) diabéticos suplementados con una dieta hipercalórica. Métodos: Un grupo de animales (n=10) alimentado con dieta estándar (SD) y otro (n=10) con dieta hipercalórica (HCD) durante 1 mes. Posteriormente, ambos grupos fueron tratados con tres dosis de Estreptozotocina. La hiperglucemia se determinó durante 73 días. Se obtuvo el peso de los animales, sangre y tejido renal. Resultados: Los animales diabéticos alimentados con HCD manifestaron hiperglucemia (250-350 mg/dL) con pérdida de peso significativa (40g), disminución del filtrado glomerular (0,491mL/min) y aumento de la matriz extracelular glomerular, intersticial y cortical (36,3, 75,2 y 70,7%, respectivamente). Los animales diabéticos que fueron alimentados con SD mostraron sólo una hiperglucemia leve y un ligero aumento de la matriz extracelular glomerular, intersticial y cort