Establishment of a Cell Line from an Adenocarcinoma of the Lung Producing Carcinoembryonic Antigen (CEA) and CA19-9

MIKI, M., ISAWA, T., TESHIMA, T., ANAZAWA, Y., MAHMUD, A.M. and NUKIWA, T. Establishment of a Cell Line from an Adenocarcinoma of the Lung Producing Carcinoembryonic Antigen (CEA) and CAJ9-9. Tohoku J. Exp. Med., 1995, 175 (4), 269-278 - An adenocarcinoma cell line was established from pleural effusion of a 67-year-old woman and designated as TK-SA. The population doubling time of the cells was 100.4hr. Chromosomal analysis revealed the TK-SA to have human aneuploid chromosomes with a near-triploid mode. Ultrastructurally, the TK-SA was compatible with adenocarcinoma. The cell line was highly tumorigenic. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide dye assay demonstrated resistance of the cell line to Cis-platin (CDDP), Cyclophosphamide (CPM) and Tegafur/Uracil (UFT). High carcinoembryonic antigen (CEA) and CA19-9 levels were similar in patient's serum and conditioned medium.