Antioxidant activity by DPPH assay of potential solutions to be applied on bleached teeth

The aim of this study was to assess, using the DPPH assay, the antioxidant activity of several substances that could be proposed to immediately revert the problems caused by bleaching procedures. The percentage of antioxidant activity (AA%) of 10% ascorbic acid solution (AAcidS), 10% ascorbic acid g...

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Veröffentlicht in:Brazilian dental journal 2012, Vol.23 (1), p.22-27
Hauptverfasser: Garcia, Eugenio José, Oldoni, Tatiane Luiza Cadorin, Alencar, Severino Matias de, Reis, Alessandra, Loguercio, Alessandro D., Grande, Rosa Helena Miranda
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container_end_page 27
container_issue 1
container_start_page 22
container_title Brazilian dental journal
container_volume 23
creator Garcia, Eugenio José
Oldoni, Tatiane Luiza Cadorin
Alencar, Severino Matias de
Reis, Alessandra
Loguercio, Alessandro D.
Grande, Rosa Helena Miranda
description The aim of this study was to assess, using the DPPH assay, the antioxidant activity of several substances that could be proposed to immediately revert the problems caused by bleaching procedures. The percentage of antioxidant activity (AA%) of 10% ascorbic acid solution (AAcidS), 10% ascorbic acid gel (AAcidG), 10% sodium ascorbate solution (SodAsS), 10% sodium ascorbate gel (SodAsG), 10% sodium bicarbonate (Bicarb), Neutralize® (NE), Desensibilize® (DES), catalase C-40 at 10 mg/mL (CAT), 10% alcohol solution of alpha-tocopherol (VitE), Listerine® (LIS), 0.12% chlorhexidine (CHX), Croton Lechleri (CL), 10 % aqueous solution of Uncaria Tomentosa (UT), artificial saliva (ArtS) and 0.05% sodium fluoride (NaF) was assessed in triplicate by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical assay. All substances exhibited antioxidant activity, except for CL. AAcidS, AAcidG and VitE exhibited the highest AA% (p
doi_str_mv 10.1590/S0103-64402012000100004
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The percentage of antioxidant activity (AA%) of 10% ascorbic acid solution (AAcidS), 10% ascorbic acid gel (AAcidG), 10% sodium ascorbate solution (SodAsS), 10% sodium ascorbate gel (SodAsG), 10% sodium bicarbonate (Bicarb), Neutralize® (NE), Desensibilize® (DES), catalase C-40 at 10 mg/mL (CAT), 10% alcohol solution of alpha-tocopherol (VitE), Listerine® (LIS), 0.12% chlorhexidine (CHX), Croton Lechleri (CL), 10 % aqueous solution of Uncaria Tomentosa (UT), artificial saliva (ArtS) and 0.05% sodium fluoride (NaF) was assessed in triplicate by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical assay. All substances exhibited antioxidant activity, except for CL. AAcidS, AAcidG and VitE exhibited the highest AA% (p&lt;0.05). On the contrary, CHX, NE, LIS and NaF showed the lowest AA% (p&lt;0.05). In conclusion, AAcidS, AAcidG, SodAsS, SodAsG and VitE presented the highest antioxidant activity among substances tested in this study. The DPPH assay provides an easy and rapid way to evaluate potential antioxidants. O objetivo deste estudo foi avaliar, por meio do DPPH, a atividade antioxidante de substâncias que poderiam ser propostas para reverter de imediato os problemas causados pelos procedimentos de clareamento. A porcentagem de atividade antioxidante (AA%) da solução de acido ascórbico 10% (AAcidS), gel de ácido ascórbico a 10% (AAcidG), solução de ascorbato de sódio 10% (SodAsS), gel de ascorbato de sódio 10% (SodAsG), bicarbonato de sódio 10% (Bicarb), Neutralize® (NE), Desensibilize® (DES), catalase C-40 10 mg/mL (CAT), solução alcoólica 10% de alfa-tocoferol (VitE), Listerine® (LIS), clorexidina 0,12% (CHX), CrotonLechleri (CL), solução aquosa 10% de Uncaria Tomentosa (UT), saliva artificial (ArtS) e fluoreto de sódio 0,05% (NaF) foi avaliada em triplicata pelo teste de radicais livres 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH). Todas as substâncias apresentaram atividade antioxidante, exceto a CL. AAcidS, AAcidG e VitE mostraram os maiores valores de AA% (p&lt;0,05). Por outro lado, CHX, NE, LIS e NaF mostraram os valores mais baixos de AA% (p&lt;0,05). Em conclusão, AAcidS, AAcidG, SodAsS, SodAsG e VitE apresentaram os mais altos valores de atividade antioxidante entre as substâncias testadas. 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The percentage of antioxidant activity (AA%) of 10% ascorbic acid solution (AAcidS), 10% ascorbic acid gel (AAcidG), 10% sodium ascorbate solution (SodAsS), 10% sodium ascorbate gel (SodAsG), 10% sodium bicarbonate (Bicarb), Neutralize® (NE), Desensibilize® (DES), catalase C-40 at 10 mg/mL (CAT), 10% alcohol solution of alpha-tocopherol (VitE), Listerine® (LIS), 0.12% chlorhexidine (CHX), Croton Lechleri (CL), 10 % aqueous solution of Uncaria Tomentosa (UT), artificial saliva (ArtS) and 0.05% sodium fluoride (NaF) was assessed in triplicate by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical assay. All substances exhibited antioxidant activity, except for CL. AAcidS, AAcidG and VitE exhibited the highest AA% (p&lt;0.05). On the contrary, CHX, NE, LIS and NaF showed the lowest AA% (p&lt;0.05). In conclusion, AAcidS, AAcidG, SodAsS, SodAsG and VitE presented the highest antioxidant activity among substances tested in this study. The DPPH assay provides an easy and rapid way to evaluate potential antioxidants. O objetivo deste estudo foi avaliar, por meio do DPPH, a atividade antioxidante de substâncias que poderiam ser propostas para reverter de imediato os problemas causados pelos procedimentos de clareamento. A porcentagem de atividade antioxidante (AA%) da solução de acido ascórbico 10% (AAcidS), gel de ácido ascórbico a 10% (AAcidG), solução de ascorbato de sódio 10% (SodAsS), gel de ascorbato de sódio 10% (SodAsG), bicarbonato de sódio 10% (Bicarb), Neutralize® (NE), Desensibilize® (DES), catalase C-40 10 mg/mL (CAT), solução alcoólica 10% de alfa-tocoferol (VitE), Listerine® (LIS), clorexidina 0,12% (CHX), CrotonLechleri (CL), solução aquosa 10% de Uncaria Tomentosa (UT), saliva artificial (ArtS) e fluoreto de sódio 0,05% (NaF) foi avaliada em triplicata pelo teste de radicais livres 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH). Todas as substâncias apresentaram atividade antioxidante, exceto a CL. AAcidS, AAcidG e VitE mostraram os maiores valores de AA% (p&lt;0,05). Por outro lado, CHX, NE, LIS e NaF mostraram os valores mais baixos de AA% (p&lt;0,05). Em conclusão, AAcidS, AAcidG, SodAsS, SodAsG e VitE apresentaram os mais altos valores de atividade antioxidante entre as substâncias testadas. 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The percentage of antioxidant activity (AA%) of 10% ascorbic acid solution (AAcidS), 10% ascorbic acid gel (AAcidG), 10% sodium ascorbate solution (SodAsS), 10% sodium ascorbate gel (SodAsG), 10% sodium bicarbonate (Bicarb), Neutralize® (NE), Desensibilize® (DES), catalase C-40 at 10 mg/mL (CAT), 10% alcohol solution of alpha-tocopherol (VitE), Listerine® (LIS), 0.12% chlorhexidine (CHX), Croton Lechleri (CL), 10 % aqueous solution of Uncaria Tomentosa (UT), artificial saliva (ArtS) and 0.05% sodium fluoride (NaF) was assessed in triplicate by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical assay. All substances exhibited antioxidant activity, except for CL. AAcidS, AAcidG and VitE exhibited the highest AA% (p&lt;0.05). On the contrary, CHX, NE, LIS and NaF showed the lowest AA% (p&lt;0.05). In conclusion, AAcidS, AAcidG, SodAsS, SodAsG and VitE presented the highest antioxidant activity among substances tested in this study. The DPPH assay provides an easy and rapid way to evaluate potential antioxidants. O objetivo deste estudo foi avaliar, por meio do DPPH, a atividade antioxidante de substâncias que poderiam ser propostas para reverter de imediato os problemas causados pelos procedimentos de clareamento. A porcentagem de atividade antioxidante (AA%) da solução de acido ascórbico 10% (AAcidS), gel de ácido ascórbico a 10% (AAcidG), solução de ascorbato de sódio 10% (SodAsS), gel de ascorbato de sódio 10% (SodAsG), bicarbonato de sódio 10% (Bicarb), Neutralize® (NE), Desensibilize® (DES), catalase C-40 10 mg/mL (CAT), solução alcoólica 10% de alfa-tocoferol (VitE), Listerine® (LIS), clorexidina 0,12% (CHX), CrotonLechleri (CL), solução aquosa 10% de Uncaria Tomentosa (UT), saliva artificial (ArtS) e fluoreto de sódio 0,05% (NaF) foi avaliada em triplicata pelo teste de radicais livres 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH). Todas as substâncias apresentaram atividade antioxidante, exceto a CL. AAcidS, AAcidG e VitE mostraram os maiores valores de AA% (p&lt;0,05). Por outro lado, CHX, NE, LIS e NaF mostraram os valores mais baixos de AA% (p&lt;0,05). Em conclusão, AAcidS, AAcidG, SodAsS, SodAsG e VitE apresentaram os mais altos valores de atividade antioxidante entre as substâncias testadas. O teste DPPH é um método rápido e fácil para avaliar o potencial antioxidante.</abstract><doi>10.1590/S0103-64402012000100004</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
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title Antioxidant activity by DPPH assay of potential solutions to be applied on bleached teeth
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