A retrospective search for bovine respiratory syncytial virus (BRSV) antigens in histological specimens by immunofluorescence and immunohistochemistry

Bovine respiratory syncytial virus (BRSV) has been only sporadically identified as a causative agent of respiratory disease in Brazil. This contrasts with frequent reports of clinical and histopathological findings suggestive of BRSV-associated disease. In order to examine a possible involvement of...

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Veröffentlicht in:Pesquisa Veterinária Brasileira 2000-12, Vol.20 (4), p.139-143
Hauptverfasser: Flores, Eduardo F.(Universidade Federal de Santa Maria Departamento de Medicina Veterinária Preventiva e Depto Microbiologia e Parasitologia), Weiblen, Rudi(Universidade Federal de Santa Maria Departamento de Medicina Veterinária Preventiva e Depto Microbiologia e Parasitologia), Medeiros, Margareti(Universidade Federal de Santa Maria Medicina Veterinária), Botton, Sônia A.(Universidade Federal de Santa Maria Departamento de Medicina Veterinária Preventiva e Depto Microbiologia e Parasitologia), Irigoyen, Luiz F.(Universidade Federal de Santa Maria Departamento de Patologia), Driemeier, David(Universidade Federal do Rio Grande do Sul Faculdade de Veterinária), Schuch, Luis F.(Universidade Federal de Pelotas Faculdade de Veterinária), Moraes, Mauro(Universidade Federal de Viçosa Departamento de Medicina Veterinária)
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Zusammenfassung:Bovine respiratory syncytial virus (BRSV) has been only sporadically identified as a causative agent of respiratory disease in Brazil. This contrasts with frequent reports of clinical and histopathological findings suggestive of BRSV-associated disease. In order to examine a possible involvement of BRSV in cases of calf pneumonia, a retrospective search was performed for BRSV antigens in histological specimens submitted to veterinary diagnostic services from the states of Rio Grande do Sul and Minas Gerais. Ten out of 41 cases examined (24.4%) were positive for BRSV antigens by immunohistochemistry (IPX). Eight of these cases (19.5%) were also positive by indirect immunofluorescence (IFA), and 31 cases (75.6%) were negative in both assays. In the lungs, BRSV antigens were predominantly observed in epithelial cells of bronchioles and less frequently found in alveoli. In one case, antigens were detected only in the epithelium of the alveolar septae. The presence of antigen-positive cells was largely restricted to epithelial cells of these airways. In two cases, positive staining was also observed in cells and cellular debris in the exudate within the pulmonary airways. The clinical cases positive for BRSV antigens were observed mainly in young animals (2 to 12 month-old) from dairy herds. The main microscopic changes included bronchointerstitial pneumonia characterized by thickening of alveolar septae adjacent to airways by mononuclear cell infiltrates, and the presence of alveolar syncytial giant cells. In summary, the results demonstrate the suitability of the immunodetection of viral antigens in routinely fixed tissue specimens as a diagnostic tool for BRSV infection. Moreover, the findings provide further evidence of the importance of BRSV as a respiratory pathogen of young cattle in southeastern and southern Brazil. O Vírus Respiratório Sincicial Bovino (BRSV) tem sido raramente identificado como agente etiológico de doença respiratória em bovinos no Brasil. Isso contrasta com freqüentes relatos clínicos e histopatológicos sugestivos de enfermidade associada ao BRSV. Com o objetivo de investigar o possível envolvimento do BRSV em casos de pneumonia em bovinos jovens, realizou-se uma pesquisa retrospectiva para antígenos do BRSV em cortes histológicos de materiais submetidos a quatro serviços de diagnóstico nos Estados do Rio Grande do Sul e Minas Gerais. Dez entre 41 casos examinados (24,4%) foram positivos para antígenos do BRSV por imunohistoquímica (
ISSN:0100-736X
1678-5150
0100-736X
1678-5150
DOI:10.1590/S0100-736X2000000400002