Analysis of the presence and location of mast cells in periapical cysts and periapical granulomas
ABSTRACT Objective: The aim of the present study was to locate mast cells in chronic periapical lesions (granulomas and cysts) by using histochemical techniques and toluidine blue staining. Methods: A quantitative, descriptive, cross-sectional and retrospective research was performed. The sample was...
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Veröffentlicht in: | RGO - Revista Gaúcha de Odontologia 2016-12, Vol.64 (4), p.376-381 |
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Zusammenfassung: | ABSTRACT Objective: The aim of the present study was to locate mast cells in chronic periapical lesions (granulomas and cysts) by using histochemical techniques and toluidine blue staining. Methods: A quantitative, descriptive, cross-sectional and retrospective research was performed. The sample was obtained from histopathological reports in the archives of the laboratory of surgical pathology of the University of Pernambuco between November 2014 and May 2015. Results: Sixteen cases of granuloma and 21 cases of periapical cysts were selected. The stained slides were analyzed by two examiners at different times, in a double-blind study. Mast cells were found in 13 (61.9%) of the periapical cyst cases, located in the capsule of the lesion. In the periapical granuloma cases, mast cells were found in eight cases (50%), located in the granulation tissue. Conclusion: Mast cells were detected in both cysts and periapical granuloma, located in the capsule and granulation tissue, respectively. Mast cells were more prevalent in periapical cysts than in periapical granuloma.
RESUMO Objetivo: Localizar mastócitos em lesões periapicais (granulomas e cistos) através de técnicas histoquímicas e corante azul de toluidina. Métodos: Uma pesquisa quantitativa, descritiva, transversal e retrospectiva foi realizada. As amostras foram obtidas de documentos histopatológicos nos arquivos do laboratório de patologia cirúrgica da Universidade de Pernambuco entre Novembro de 2014 e Março de 2015. Resultados: Dezesseis casos de granuloma e 21 casos de cistos periapicales foram selecionados. As lâminas coradas foram analisadas por dois examinadores em tempos diferentes, em um estudo duplo-cego. Mastócitos foram encontrados em 13 (61,9%) dos casos de cistos periapicales, localizados na cápsula da lesão. Nos granulomas periapicais, mastócitos foram encontrados em oito casos (50%), localizados no tecido de granulação. Conclusão: Mastócitos foram detectados tanto em cistos quanto em granulomas periapicais, localizados na cápsula e no tecido de granulação respectivamente. Os mastócitos estavam mais presentes nos cistos do que nos granulomas periapicais. |
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ISSN: | 0103-6971 1981-8637 1981-8637 |
DOI: | 10.1590/1981-863720160003000023113 |