Chronic Periodontitis and RANKL/OPG Ratio in Peri-Implant Mucosae Inflammation

Abstract tHistory of chronic periodontitis (CP) is a risk factor for oseointegration failure. The osteoclastogenesis system (RANK, RANKL and OPG) is critical for bone homeostatic control. We investigated the levels of OPG and RANKL in peri-implant tissues from volunteers with and without a history o...

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Veröffentlicht in:Brazilian dental journal 2018-02, Vol.29 (1), p.14-22
Hauptverfasser: Costa, Lucas Carneiro, Fonseca, Marcos Alexandre da, Pinheiro, Aristides da Rosa, Aguiar, Telma Regina da Silva, Machado, Aldir Nascimento, Quinelato, Valquiria, Bonato, Leticia Ladeira, Aguiar, Diego Pinheiro, Vieira, Thays, Almeida, Fernando Luiz Duarte de, Lobo, Julie Calixto, Jordão, Miriam, Lomardo, Priscilla Gonçalves, Granjeiro, José Mauro, Casado, Priscila Ladeira
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Sprache:eng
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Zusammenfassung:Abstract tHistory of chronic periodontitis (CP) is a risk factor for oseointegration failure. The osteoclastogenesis system (RANK, RANKL and OPG) is critical for bone homeostatic control. We investigated the levels of OPG and RANKL in peri-implant tissues from volunteers with and without a history of CP and their association with mucosae inflammation. This is a single-blind case-contro study. Diagnosis of a history of CP and peri-implant examination was performed on 46 volunteers, divided into control (without history of CP, n=26) and CP group (with history of CP, n=20). Gingival biopsies were harvested during implant exposure. Quantitative PCR evaluated OPG/RANKL mRNA expressions. OPG and RANKL proteins were analyzed by western blot and immunohistochemistry assay. The chi-square test analyzed the significance of nominal variables between groups while continuous variables were analyzed by T-test or Mann-Whitney test, after Shapiro-Wilk test evaluation. The 2-ΔΔCT Livak method calculation evaluated the gene expression. Values of p
ISSN:0103-6440
1806-4760
DOI:10.1590/0103-6440201801241