Characterization of an Expressed Triticum monococcum Glu-A1y Gene Containing a Premature Termination Codon in its C-terminal Coding Region
Premature termination codons (PTCs) are an important reason for the silence of high-molecular-weight glutenin subunits in Triticum species. Although the Glu-A1y gene is generally silent in common wheat, we here isolated an expressed Glu-A1y gene containing a PTC, named 1Ay8.3 , from Triticum monococ...
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Veröffentlicht in: | Cereal research communications 2018-06, Vol.46 (2), p.201-210 |
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Sprache: | eng |
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Zusammenfassung: | Premature termination codons (PTCs) are an important reason for the silence of high-molecular-weight glutenin subunits in
Triticum
species. Although the
Glu-A1y
gene is generally silent in common wheat, we here isolated an expressed
Glu-A1y
gene containing a PTC, named
1Ay8.3
, from
Triticum monococcum
ssp.
monococcum
(A
m
A
m
, 2n = 2x = 14). Despite the presence of a PTC (TAG) at base pair positions 1879–1881 in the C-terminal coding region, this did not obviously affect
1Ay8.3
expression in seeds. This was demonstrated by the fact that when the PTC TAG of
1Ay8.3
was mutated to the CAG codon, the mutant in
Escherichia coli
bacterial cells expressed the same subunit as in the seeds. However, in
E. coli
,
1Ay8.3
containing the PTC expressed a truncated protein with faster electrophoretic mobility than that in seeds, suggesting that PTC translation termination suppression probably occurs
in vivo
(seeds) but not
in vitro
(
E. coli
). This may represent one of only a few reports on the PTC termination suppression phenomenon in genes. |
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ISSN: | 0133-3720 1788-9170 |
DOI: | 10.1556/0806.46.2018.02 |