Molecular cloning of the restriction fragments derived from double EcoRI/PstI digestion of the calf satellite I DNA and their restriction analysis

Calf satellite I DNA digested with EcoRI and PstI gives three fragments 643, 621 and 83 bp long. Two of them , the 643 and 621 bp were cloned using pBR 322 vector and analyzed by means of the MspI and Haelll restriction enzymes.

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Bibliographische Detailangaben
Veröffentlicht in:	Zeitschrift für Naturforschung C. A journal of biosciences 1979-12, Vol.34 (12), p.1151-1155
Hauptverfasser:	Klysik, J, Furtak, K, Szymczak, G, Bartnik, E, Skowronski, J, Panusz, H
Format:	Artikel
Sprache:	eng
Schlagworte:	animal physiology Animals Calf Satellite I DNA Cattle Cloning Cloning, Molecular Deoxyribonucleases, Type II Site-Specific DNA Restriction Enzymes DNA, Recombinant - metabolism DNA, Satellite - metabolism Electrophoresis, Polyacrylamide Gel Mapping Molecular Weight Restriction
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Beschreibung
Zusammenfassung:	Calf satellite I DNA digested with EcoRI and PstI gives three fragments 643, 621 and 83 bp long. Two of them , the 643 and 621 bp were cloned using pBR 322 vector and analyzed by means of the MspI and Haelll restriction enzymes.
ISSN:	0341-0382 0939-5075 1865-7125
DOI:	10.1515/znc-1979-1212