Changes in the Macromolecular Structure of DNA-RNA-Protein Complexes Induced by Gel-Filtration on Sephadex G-25

Hydrodynamic shearing forces appearing during nucleoprotein gel-filtration on Sephadex G-25 cause a disruption of the macromolecular structure in nucleoproleins. The effect of these shearing forces can be observed both in electron micrographs and by electro­phoretic analysis. The decrease of the mol...

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Veröffentlicht in:Zeitschrift für Naturforschung. B, A journal of chemical sciences A journal of chemical sciences, 1970-04, Vol.25 (4), p.355-357
Hauptverfasser: David, C., Ghyka, Gr, Dumitrescu, Claudia
Format: Artikel
Sprache:eng
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Zusammenfassung:Hydrodynamic shearing forces appearing during nucleoprotein gel-filtration on Sephadex G-25 cause a disruption of the macromolecular structure in nucleoproleins. The effect of these shearing forces can be observed both in electron micrographs and by electro­phoretic analysis. The decrease of the molecular weight and also the changes in the electronegative charge,mole­cular weight ratio cause an increase of the electrophoretic mobility of the gel-filtered highly poly­merized DNA and of the RNA complexed to it compared with the not gel-filtered nucleoprotein.
ISSN:0932-0776
1865-7117
DOI:10.1515/znb-1970-0404