Novel H 2 S donor proglumide-ADT-OH protects HUVECs from ox-LDL-induced injury through NF-κB and JAK/SATA pathway

As a gaseous mediator, hydrogen sulfide (H 2 S) has many physiological effects and pathological effects in atherosclerosis. In recent years, many exogenous H 2 S donors have been synthesized to study atherosclerosis diseases. In this study, proglumide-(5-(4-hydroxyphenyl)-3 H -1,2-dithiole-3-thione)...

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Veröffentlicht in:Open medicine (Warsaw, Poland) Poland), 2021-09, Vol.16 (1), p.1318-1327
Hauptverfasser: Ou, Xuelan, Xia, Tianqin, Yang, Chunyan, Yu, Chunlei, Zhang, Shipeng, Huang, Rong, Chen, Chuan, Zhou, Chunyang
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Sprache:eng
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Zusammenfassung:As a gaseous mediator, hydrogen sulfide (H 2 S) has many physiological effects and pathological effects in atherosclerosis. In recent years, many exogenous H 2 S donors have been synthesized to study atherosclerosis diseases. In this study, proglumide-(5-(4-hydroxyphenyl)-3 H -1,2-dithiole-3-thione) (P-A) was synthesized as a H 2 S donor. The protective effect and mechanism of P-A on HUVEC that was injured by ox-LDL were detected. The HUEVCs were affected by 100 μmol/L P-A for 24 h; the release of H 2 S was the largest. After 100 μmol/L P-A acted on HUVEC damage model for 12 h, the cell proliferation activity was the best. The results showed that P-A can downregulate the expression of p-NF-кBp65 protein and reduce the amount of TNF-α and IL-6 and promote the formation of IL-10 by inhibiting the NF-кB pathway, and also induce the expression of superoxide dismutase (SOD) to protect HUVEC from ox-LDL injury. P-A can also regulate JAK/STAT pathway to reduce the expression of p-JAK2 protein and reduce the production of IL-6 and TNF-α. P-A has protective effect on HUVEC injured by ox-LDL, and the protective mechanism is related to the regulation of JAK/STAT pathway and NF-кB pathway.
ISSN:2391-5463
2391-5463
DOI:10.1515/med-2021-0287