Evaluation of sample integrity measurement with VITROS® 5,1 FS chemistry system / Evaluation der Messung der Probenintegrität am VITROS® 5,1 FS

The measurement of sample integrity on a clinical laboratory system may help to avoid misleading results. The VITROS® 5,1 FS Chemistry System is a new analyzer which offers this feature. Approximately 50 diagnostic specimens were evaluated for the presence of hemolysis, icterus, and turbidity using MicroSensor™ technology onboard the VITROS® 5,1 FS. The samples were also assessed for hemoglobin concentration on a Lange photometer using the Harboe method and values were compared with the MicroSensor™ technology hemolysis index (H-index). For the icterus index (I-index), comparison was based on total bilirubin measured on the VITROS® system. To correlate the turbidity index (T-index), a comparison with triglycerides measured on the VITROS® 5,1 FS was undertaken. In addition, samples run on the VITROS® 5,1 FS were measured on a manual photometer at 700 nm. Good correlation was established between the manual Harboe method for measurement of hemoglobin and the VITROS® 5,1 FS H-index. For the I-index, a good correlation was observed compared to the quantitative bilirubin (TBIL slide) measurement. A poor correlation was expected for the comparison of the T-index with the triglycerides as measured on the VITROS® system. An additional study comparing the VITROS® MicroSensor T-index vs. manual photometer measurement at 700 nm yielded a very good correlation. The VITROS® 5,1 FS chemistry system MicroSensor™ technology measures the index for hemolyzed, icteric, and turbid samples. The indices correlate well compared to other methods for measuring these indices. Therefore, the use of MicroSensor™ technology offers a useful feature to identify samples containing optical interferents during routine work and to avoid misleading results by providing flags. Die Messung der Probenintegrität auf einem klinisch-chemischen Laborsystem kann helfen, irreführende Ergebnisse zu vermeiden. Das VITROS 5,1 FS-Analysensystem ist ein neuer Analysator, der diese Messungen automatisch durchführt. Etwa 50 Patientenproben wurden auf hämolytische und ikterische Färbung sowie auf Trübung mit Hilfe der sogenannten MicroSensor-Technologie am VITROS 5,1 FS geprüft. Die Hämoglobin-Konzentration wurde photometrisch, unter Einsatz der Harboe-Methode ermittelt, während die MicroSensor-Technologie den Hämolyse-Index (H-Index) erfasste. Für die ikterische Färbung wurde der I-Index mittels der MicroSensor-Messung bestimmt und mit der Gesamt-bilirubin-Konzentration (Diazo-Methode) verglichen. Der Grad d