Ultrashort ssDNA in Retinoblastoma Patients Blood Plasma Detected by a Novel High Resolution HPLC Technique: a Preliminary Report
A significant population of ultrashort (50–150 n ) single-stranded DNA fragments were found in exosome-free blood plasma of retinoblastoma patients (6.84 ng mL −1 ), but not in plasma of healthy donors. An original high resolution HPLC technique has been proposed to reveal and characterize this pecu...
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| Veröffentlicht in: | Acta medica Lékarskí fakulty Univerzity Karlovy v Hradci Králove 2019, Vol.62 (4), p.170-173 |
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| container_title | Acta medica Lékarskí fakulty Univerzity Karlovy v Hradci Králove |
| container_volume | 62 |
| creator | Ermakov, Kirill V. Bukhvostov, Alexander A. Vedenkin, Alexander S. Stovbun, Sergey V. Dvornikov, Anton S. Kuznetsov, Dmitry A. |
| description | A significant population of ultrashort (50–150
n
) single-stranded DNA fragments were found in exosome-free blood plasma of retinoblastoma patients (6.84 ng mL
−1
), but not in plasma of healthy donors. An original high resolution HPLC technique has been proposed to reveal and characterize this peculiarity. To solve this task, a novel molecular size exclusion – anion exchange analytical technique was developed. Its applicability to diagnostics and oncogenesis research is quizzed here. |
| doi_str_mv | 10.14712/18059694.2020.8 |
| format | Article |
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n
) single-stranded DNA fragments were found in exosome-free blood plasma of retinoblastoma patients (6.84 ng mL
−1
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−1
), but not in plasma of healthy donors. An original high resolution HPLC technique has been proposed to reveal and characterize this peculiarity. To solve this task, a novel molecular size exclusion – anion exchange analytical technique was developed. Its applicability to diagnostics and oncogenesis research is quizzed here.</abstract><doi>10.14712/18059694.2020.8</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1211-4286 |
| ispartof | Acta medica Lékarskí fakulty Univerzity Karlovy v Hradci Králove, 2019, Vol.62 (4), p.170-173 |
| issn | 1211-4286 1805-9694 |
| language | eng |
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| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| title | Ultrashort ssDNA in Retinoblastoma Patients Blood Plasma Detected by a Novel High Resolution HPLC Technique: a Preliminary Report |
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