Ultrashort ssDNA in Retinoblastoma Patients Blood Plasma Detected by a Novel High Resolution HPLC Technique: a Preliminary Report
A significant population of ultrashort (50–150 n ) single-stranded DNA fragments were found in exosome-free blood plasma of retinoblastoma patients (6.84 ng mL −1 ), but not in plasma of healthy donors. An original high resolution HPLC technique has been proposed to reveal and characterize this pecu...
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| Veröffentlicht in: | Acta medica Lékarskí fakulty Univerzity Karlovy v Hradci Králove 2019, Vol.62 (4), p.170-173 |
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| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
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| Zusammenfassung: | A significant population of ultrashort (50–150
n
) single-stranded DNA fragments were found in exosome-free blood plasma of retinoblastoma patients (6.84 ng mL
−1
), but not in plasma of healthy donors. An original high resolution HPLC technique has been proposed to reveal and characterize this peculiarity. To solve this task, a novel molecular size exclusion – anion exchange analytical technique was developed. Its applicability to diagnostics and oncogenesis research is quizzed here. |
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| ISSN: | 1211-4286 1805-9694 |
| DOI: | 10.14712/18059694.2020.8 |