СHROMATOGRAPHIC ANALYSIS OF СYCLOSIVERSIOSIDE F

The plants Astragalus pterocephalus growing in Uzbekistan are a source of triterpene glycosides. The main triterpene glycoside, in terms of content, is a cycloartan glycoside – cyclosiversioside F. To obtain an individual biologically active compound cyclosiversioside F with 95% purity, a proposed method involves extraction with methanol, concentration and dilution with an equal volume of water, then followed by a sequential extraction from the aqueous extract with chloroform, ethyl acetate and butanol. Then a chromatographic separation of the purified amount of extractives on a column with silica gel, isolation of the substance and precipitation from a solvent system must be performed, followed by recrystallization and drying. The optimal conditions for the isolation and separation of the amount of extractive substances have been developed in order to obtain an individual glycoside. Cyclosiversioside F was authenticated by TLC in comparison with an authentic sample. Quantitative analysis of the glycoside was carried out by HPLC. The purity of cyclosiversioside F was confirmed by taking 1H and 13C NMR spectra.