Resolution Enhancement in IR Spectra of Carbohydrates by the Deconvolution Method and Comparison of the Results with Low-Temperature Spectra

This paper presents infrared (IR) spectra of β-d-glucose, cellobiose, and cellulose in the 1500–850 cm−1 range as well as the results of their deconvolution. Comparison of the results of deconvolution with the IR spectra of the investigated compounds obtained at room and helium temperatures of the sample as well as with the theoretical data has been made. It is shown that the use of the deconvolution method provides a better—compared to the low-temperature spectra—resolution of individual absorption band components for all the compounds being analyzed, especially for the polysaccharide cellulose. The results of deconvolution of IR spectra of monosaccharide β-d-glucose and disaccharide cellobiose are in good agreement with the data of normal coordinate analysis. It has been found that the minimum values of band halfwidths in the room-temperature IR spectra of β-d-glucose and cellobiose approximately coincide, while for cellulose they increase by a factor of about 1.5.