Simple and Validated LC Method for the Simultaneous Determination of Three Compounds in Mikania laevigata Extracts

The Mikania genus is widely known as guaco and is used to treat fever, rheumatism, influenza and respiratory diseases. This article deals with the simultaneous quantification of three commercially available phenolic markers (o-coumaric acid, coumarin and syringaldehyde) in M. laevigata extracts, through LC-PDA. The validation data show that the method is specific, accurate, precise and robust, and also indicative of the stability of guaco extract. The method was linear, over a range of 1.25-20.0 μg mL⁻¹ for o-coumaric acid, 2.5-40.0 μg mL⁻¹ for coumarin, and 0.25-4.0 μg mL⁻¹ for syringaldehyde. The range of recovery was 94.3-96.4% for all the components, at a level of 100%.