Stability-Indicating LC Method for Assay of Cholecalciferol

Stability-Indicating LC Method for Assay of Cholecalciferol

This paper discusses the development of a stability-indicating reversed-phase LC method for analysis of cholecalciferol as the bulk drug and in formulations. The mobile phase was acetonitrile-methanol-water 50:50:2 (v/v). The calibration plot for the drug was linear in the range 0.4-10 μg mL⁻¹. The...

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Veröffentlicht in:Chromatographia 2009-02, Vol.69 (3-4), p.385-388
Hauptverfasser: Desai, Namita D, Singh, Pirthipal P, Amin, Purnima D, Jain, Satishkumar P
Format: Artikel
Sprache:eng
Schlagworte:
Analysis
Analytical Chemistry
Biological and medical sciences
Chemistry
Chemistry and Materials Science
Chromatography
General pharmacology
Laboratory Medicine
Limited Short Communication
Medical sciences
Pharmacology. Drug treatments
Pharmacy
Proteomics
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Zusammenfassung:This paper discusses the development of a stability-indicating reversed-phase LC method for analysis of cholecalciferol as the bulk drug and in formulations. The mobile phase was acetonitrile-methanol-water 50:50:2 (v/v). The calibration plot for the drug was linear in the range 0.4-10 μg mL⁻¹. The method was accurate and precise with limits of detection and quantitation of 64 and 215 ng, respectively. Mean recovery was 100.71%. The method was used for analysis of cholecalciferol in pharmaceutical formulations in the presence of its degradation products and commonly used excipients.
ISSN:0009-5893
1612-1112
DOI:10.1365/s10337-008-0914-x