RP-LC Determination of Rutin in Amaranthus spinosus Linn. Whole Plant Powder

A simple, reproducible and efficient reverse phase high performance liquid chromatographic method has been developed for the determination of rutin in whole plant powder of Amaranthus spinosus Linn. The analysis was done with Waters Cosmosil C₁₈ column (150 x 4.6 mm i.d., 5 μm particle size) as stationary phase at a wavelength of 361 nm for detection and determination. The proposed HPLC method was validated for linearity, accuracy, precision and limit of quantitation.