On the label-free analysis of white blood cells by holographic quantitative phase imaging flow cytometry

This study presents an innovative methodology to analyze a blood sample from a healthy donor, providing a quantitative characterization of white blood cells (WBCs). It aims to evaluate the effectiveness of holographic quantitative phase imaging (QPI) flow cytometry (FC) in examining phase-contrast m...

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Veröffentlicht in:Journal of the Optical Society of America. A, Optics, image science, and vision Optics, image science, and vision, 2024-12, Vol.41 (12), p.2421
Hauptverfasser: Giugliano, Giusy, Pirone, Daniele, Behal, Jaromir, Wang, Zhe, Cerbone, Vincenza, Mugnano, Martina, Licitra, Fabrizio, Montella, Annalaura, Scalia, Giulia, Capasso, Mario, Iolascon, Achille, Mari, Silvia, Ferranti, Francesca, Bianco, Vittorio, Maffettone, Pier Luca, Memmolo, Pasquale, Miccio, Lisa, Ferraro, Pietro
Format: Artikel
Sprache:eng
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Zusammenfassung:This study presents an innovative methodology to analyze a blood sample from a healthy donor, providing a quantitative characterization of white blood cells (WBCs). It aims to evaluate the effectiveness of holographic quantitative phase imaging (QPI) flow cytometry (FC) in examining phase-contrast maps at the cellular level, thereby enabling the identification and classification of granulocyte types. Additionally, we demonstrate that an unsupervised method can differentiate granulocyte sub-types, i.e., neutrophils and eosinophils. The results instill strong confidence in the potential future use of QPI FC for liquid biopsies and/or for assessing the heterogeneity of WBCs and, more broadly, to facilitate label-free blood tests.
ISSN:1084-7529
1520-8532
DOI:10.1364/JOSAA.536841