On the label-free analysis of white blood cells by holographic quantitative phase imaging flow cytometry
This study presents an innovative methodology to analyze a blood sample from a healthy donor, providing a quantitative characterization of white blood cells (WBCs). It aims to evaluate the effectiveness of holographic quantitative phase imaging (QPI) flow cytometry (FC) in examining phase-contrast m...
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Veröffentlicht in: | Journal of the Optical Society of America. A, Optics, image science, and vision Optics, image science, and vision, 2024-12, Vol.41 (12), p.2421 |
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Hauptverfasser: | , , , , , , , , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
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Zusammenfassung: | This study presents an innovative methodology to analyze a blood sample from a healthy donor, providing a quantitative characterization of white blood cells (WBCs). It aims to evaluate the effectiveness of holographic quantitative phase imaging (QPI) flow cytometry (FC) in examining phase-contrast maps at the cellular level, thereby enabling the identification and classification of granulocyte types. Additionally, we demonstrate that an unsupervised method can differentiate granulocyte sub-types, i.e., neutrophils and eosinophils. The results instill strong confidence in the potential future use of QPI FC for liquid biopsies and/or for assessing the heterogeneity of WBCs and, more broadly, to facilitate label-free blood tests. |
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ISSN: | 1084-7529 1520-8532 |
DOI: | 10.1364/JOSAA.536841 |