Preparation of Gelatin Particles for Use in Passive Agglutination Immunoassay

Artificial support material for use in passive agglutination immunoassay was prepared from the complex coacervation of gelatin and gum arabic. The gelatin particles with a diameter of 2.5 gm and with a zeta potential of-15 mV at an ionic strength of 0.10, pH 7.2 were appropriate for use in passive agglutination immunoassay. Red, blue, and purple colored-gelatin particles were obtained by changing the dye. Thus, the color, size, and electrophoretic mobility of the gelatin particles prepared from the complex coacervation tecnique were controlled to some extent and the gelatin particles were found to be suitable for use in passive agglutination immunoassays.