Studies on the Incorporation of Enzyme by Bacterial Cell Part II

In an effort to learn more about the basic reasons for inactivation mechanism of enzymes by Bacilluscirculans Jordan strain 1-3, OMT 6301, the effect of temperature, kind of buffers, pH or concentration of buffers and concentration of the cells or enzymes were studied. Temperature optimum was around at 30 to 35°C. Phosphate buffer was essential for this phenomenon. Its pH optimum was 7.0 to 7.5 and concentration optimum was 20 mM to 40 mM. The rate which enzymes were inactivated by the cells was nearly proportional to the concentration of cells within this experimental condition but no effect with concentration of enzymes. This ability of the cells to inactivate the enzymes in the reaction media adaptively increased.