Single microspore reverse transcription-polymerase chain reaction: Dynamic change of gene expression during microsporogenesis

A sensitive and reliable single microspore reverse transcription-polymerase chain reaction (RT-PCR) assay was developed that requires little sample material and only a short sampling time and ensures template preservation. We propose that the assay provides a useful tool to study not only cell-cycle...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2005, Vol.69 (11), p.2240-2243
Hauptverfasser:	Zhou, B.(Wuhan Univ. (China)), Sauter, M, Sun, M
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Cell Division - genetics ESTERILIDAD MASCULINA EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE EXPRESSION gene expression dynamics Gene Expression Regulation, Plant INFERTILITE MALE MALE INFERTILITY MICROSCOPIA MICROSCOPIE MICROSCOPY microspores Mitosis - genetics MUTANT MUTANTES MUTANTS Oryza - cytology Oryza - genetics ORYZA SATIVA Oryza sativa L PCR Plant Cells POLEN POLLEN Pollen - genetics Pollen - growth & development Reverse Transcriptase Polymerase Chain Reaction single microspore (RT-PCR)
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Zusammenfassung:	A sensitive and reliable single microspore reverse transcription-polymerase chain reaction (RT-PCR) assay was developed that requires little sample material and only a short sampling time and ensures template preservation. We propose that the assay provides a useful tool to study not only cell-cycle related events in developing pollen but also the molecular mechanisms underlying male sterility and mutants impaired in pollen development.
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.69.2240