Neurite Outgrowth-stimulating Activities of β-Casomorphins in Neuro-2a Mouse Neuroblastoma Cells

Endogenous opioid peptides and opiate drugs are known to affect the development of the nervous system. β-Casomorphins (β-CMs) belong to a family of exogenous opioid peptides derived from the milk protein β-casein by proteolytic fragmentation. We investigated the effects of various fragments and anal...

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Veröffentlicht in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003, Vol.67 (12), p.2541-2547
Hauptverfasser: SAKAGUCHI, Minoru, MURAYAMA, Kouichi, JINSMAA, Yunden, YOSHIKAWA, Masaaki, MATSUMURA, Eiko
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Sprache:eng
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Zusammenfassung:Endogenous opioid peptides and opiate drugs are known to affect the development of the nervous system. β-Casomorphins (β-CMs) belong to a family of exogenous opioid peptides derived from the milk protein β-casein by proteolytic fragmentation. We investigated the effects of various fragments and analogues of β-CM on neurite outgrowth in Neuro-2a mouse neuroblastoma cells. The fragments β-CM-5 to -9 and β-CM-5 amide stimulated neurite outgrowth. Fragments shorter than β-CM-5 (β-CM-3, -4, and β-CM-4 amide) and longer than β-CM-9 (β-CM-13 and -21) had no effects. The activity of β-CMs to promote neurite outgrowth does not correlate with their opioid activity in guinea-pig ileum. The effect of the most potent fragment, β-CM-5, was prevented by the μ-opioid receptor-selectiveantagonist D-Phe-Cys 2 -Tyr 3 -D-Trp-Orn 5 -Thr 6 -Pen 7 - Thr 8 -NH 2 (CTOP), or by pretreatment with pertussis toxin. These results suggest that the stimulatory effects of β-CMs on neurite outgrowth were mediated through G protein-coupled μ-opioid receptors.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.67.2541