Highly Efficient Transformation of the Diatom Phaeodactylum tricornutum by Multi-Pulse Electroporation

A highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi-sequence pulses to introduce foreign DNAs into the cells. By optimizing pulse conditions, the diatom cells can be transformed without re...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2013, Vol.77 (4), p.874-876
Hauptverfasser:	MIYAHARA, Mado, AOI, Masaki, INOUE-KASHINO, Natsuko, KASHINO, Yasuhiro, IFUKU, Kentaro
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillariophyceae diatom Diatoms - cytology Diatoms - genetics electroporation Electroporation - methods Genes, Reporter - genetics multi-pulse Organisms, Genetically Modified Phaeodactylum tricornutum Transformation, Genetic
Online-Zugang:	Volltext
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