Highly Efficient Transformation of the Diatom Phaeodactylum tricornutum by Multi-Pulse Electroporation

Highly Efficient Transformation of the Diatom Phaeodactylum tricornutum by Multi-Pulse Electroporation

A highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi-sequence pulses to introduce foreign DNAs into the cells. By optimizing pulse conditions, the diatom cells can be transformed without re...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2013, Vol.77 (4), p.874-876
Hauptverfasser: MIYAHARA, Mado, AOI, Masaki, INOUE-KASHINO, Natsuko, KASHINO, Yasuhiro, IFUKU, Kentaro
Format: Artikel
Sprache:eng
Schlagworte:
Bacillariophyceae
diatom
Diatoms - cytology
Diatoms - genetics
electroporation
Electroporation - methods
Genes, Reporter - genetics
multi-pulse
Organisms, Genetically Modified
Phaeodactylum tricornutum
Transformation, Genetic
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi-sequence pulses to introduce foreign DNAs into the cells. By optimizing pulse conditions, the diatom cells can be transformed without removing rigid silica-based cell walls, and high transformation efficiency (about 4,500 per 10(8) cells) is achieved.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.120936