Analysis of Distribution and Diversity of Patatin Promoters in Solanum Species by Using the Polymerase Chain Reaction Technique

Distribution and diversity of patatin promoters in Solanum species were analyzed by using the Polymerase Chain Reaction (PCR) technique. The promoter region (-580 to + 11) of the B33 gene, one of the class I patatin genes, was cloned for use as a probe for Southern blotting. All the lines used in this experiment, even the no-tuber-bearing wild species, exhibited a homologous sequence to the class I patatin promoter region which controls the tuber-specific expression. The fragment pattern of amplified DNA varied with the lines.