In vitro evaluation of ruminal digestibility and fermentation characteristics of local feedstuff-based beef cattle ration [version 2; peer review: 1 approved with reservations]

Background: Consumption of local feedstuff Neptunia plena L. Benth and Leersia hexandra Swartz as a ration by the animal subject is expected to promote cost efficiency and production, as well as provide essential nutrition needs. Therefore, this study aimed to evaluate dry matter digestibility (DMD), organic matter digestibility (OMD), ammonia (NH 3) production, and volatile fatty acid (VFA) in beef cattle. Methods: Feed and rumen inoculum samples were prepared and analyzed for their proximate contents. There were five treatment groups based on the diet received by beef cattle, namely: T 1 ( Leersia hexandra Swartz 100 %); T 2 ( Neptunia plena L. Benth 100%); T 3 ( Leersia hexandra Swartz 15% + ( Neptunia plena L. Benth 15% + 70 % Other Feedstuffs); T 4 ( Leersia hexandra Swartz 20% + ( Neptunia plena L. Benth 20% + 60% Other Feedstuffs); T 5 ( Leersia hexandra Swartz 25% + ( Neptunia plena L. Benth 25% + 50% Other Feedstuffs). In vitro approaches were used to determine the DMD, OMD, NH 3 production, and VFA in beef cattle. The data were analyzed using ANOVA at a significance level of 95%, and a Duncan Multiple Range Test. Results: The results showed that the highest DMD (P