Creation and Characterization of the β-1,4-Glucanase (CMCase)Producing Mutant Strains of Bacillus sp. with Ultraviolet Radiation and Ethidium Bromide

In this study, the β-1,4-glucanase-producing Bacillus sp. strains were isolated from soil; then, in these strains, the undesired enzyme genes such as α-amylase, xylanase, protease were inactivated by mutation using Ethidium bromide (EtBr) and UV ray; thus, only β-1,4-glucanase producing strains were...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of the Hellenic Veterinary Medical Society 2023-04, Vol.74 (1), p.5119-5130
Hauptverfasser: Sezan, A, Baylan, M
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:In this study, the β-1,4-glucanase-producing Bacillus sp. strains were isolated from soil; then, in these strains, the undesired enzyme genes such as α-amylase, xylanase, protease were inactivated by mutation using Ethidium bromide (EtBr) and UV ray; thus, only β-1,4-glucanase producing strains were obtained.  The enzyme activities (pH, temperature, thermostability, etc.) of the wild strain and its two mutant variants (UV mutant (mUV), EtBr mutant (mEB)) producing β-1,4-glucanase were partially characterized. It was found that the optimum pHs for the mUV, mEB, and the wild strain were 7.0, 6.0 and 9.0, respectively; and the optimum temperatures were 50, 60, and 50 ℃, respectively. Finally, the molecular weights of the cellulase enzymes of all the three bacteria were found to be 40 kDa by SDS-PAGE and zymogram analysis.
ISSN:1792-2720
2585-3724
DOI:10.12681/jhvms.24694