Determination of Chlorinated Pyrenes in Soil by GC/MS

An analytical method for the determination of 1-chloropyrene and dichloropyrene in soil samples was developed. These compounds were extracted from soil with benzene-ethanol mixture (4 : 1, v/v), using ultrasonic irradiation for 10 min, and then centrifugal separation at 3000 rpm. The supernatant was concentrated to dryness and re-dissolved into 3 ml of n-hexane. This solution was cleaned up by Florisil column chromatography, using n-hexane, and then n-hexane containing 15% diethyl ether. The latter fraction was concentrated to 2 ml for the GC/MS (SIM) determination of chlorinated pyrenes. The recoveries of 1-chloropyrene and dichloropyrene were 86 and 90%, respectively. The limits of the determination were 1 ppb and 0.5 ppb, respectively. This analytical method was also applied to the soil samples collected from Yokohama and Kawasaki cities.