Studies on Relationship between Thin-Layer Chromatography and Column Chromatography. III. : Utilization of Mixed Fluorescent Substance

Utilization of the micro-column chromatography was investigated to obtain a comparable results between thin-layer chromatography and column chromatography. The developing feature in column chromatography of substances absorbing in the ultraviolet region was observed directly as a colored zone under improved ultraviolet ray source by the use of an adsorbent containing mixed fluorescent substance and a quartz tube. This method was applied to micro-column chromatography as well as to thin-layer chromatography used in qualitative experiment. Results from the micro-column chromatography were very similar to those from dry column chromatography. When dry column chromatography was to be carried out with reference to the data from thin-layer chromatography, informations on the effect of developer vapor and manner of separation were obtained by the preliminary-use of micro-column chromatography. From these results, the developer and adsorbent for sharp separation were selected.