Centrifugal Partition Chromatography (CPC) as a Useful Method for Determination of Partition Coefficients between Octanol and Water

Centrifugal partition chromatographies (CPC) of three series of compounds, phenols, benzoic acids and N-phenylanthranilic acids, were performed with acetonitrile as a mobile phase and n-hexane as a stationary phase. Values for the partition coefficient (Pcpc) of these compounds were determined from the ratio of their concentration in the n-hexane phase to that in the acetonitrile phase, and they were compared with the partition coefficient between octanol and water determined by the shaking-flask method (Poct). A significant linear relation was obtained between log Pcpc and log Poct for each series of compounds up to log Poct values of about 6, indicating that CPC is a very useful method for simple and accurate determination of Poct.