Colloidal Nanoparticles from Poly(N-isopropylacrylamide)-graft-DNA for Single Nucleotide Discrimination Based on Salt-induced Aggregation: Extension to Long Target DNA

Colloidal Nanoparticles from Poly(N-isopropylacrylamide)-graft-DNA for Single Nucleotide Discrimination Based on Salt-induced Aggregation: Extension to Long Target DNA

The DNA-linked colloidal nanoparticle was prepared through the phase transition of the amphiphilic copolymer consisting of DNA as the hydrophilic part and thermo-responsive poly(N-isopropylacrylamide) as the hydrophobic part. We have succeeded in detecting the single nucleotide difference of 39-mer...

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Veröffentlicht in:Chemistry letters 2004-12, Vol.33 (12), p.1602-1603
Hauptverfasser: Tang, Zhonglan, Takarada, Tohru, Sato, Yoshikuni, Maeda, Mizuo
Format: Artikel
Sprache:eng
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Zusammenfassung:The DNA-linked colloidal nanoparticle was prepared through the phase transition of the amphiphilic copolymer consisting of DNA as the hydrophilic part and thermo-responsive poly(N-isopropylacrylamide) as the hydrophobic part. We have succeeded in detecting the single nucleotide difference of 39-mer target DNA by using a salt-induced aggregation of the particles.
ISSN:0366-7022
1348-0715
DOI:10.1246/cl.2004.1602