The Effect of Pituitrin Injection on the Water Balance of Bufo Regularis Reuss

A method of cannulating the cloaca of Anura is described. Using this method it is found that in Bufo regular is the increase in body water which follows pituitrin injection is the result of an increased rate of water uptake, together with a diminished urine flow. The excess water is mostly retained in the lymph spaces, while the water content of the tissues of the body increases very little. The bearing of these results on the problem of the identification of the substance or substances which elicit the amphibian water balance effect, and the biological significance of the organization of the anuran lymphatic system are discussed.