A Prospective, Randomized Study of Endometrial Telomerase during the Menstrual Cycle
The purpose of this study was to characterize telomerase activity during the menstrual cycle, focusing on the luteal phase. A total of 84 endometrial biopsy samples were obtained from 72 participants. Daily urinary LH testing (OvuQuick, Quidel) was used to establish the day of the LH rise, and parti...
Gespeichert in:
Veröffentlicht in: | The journal of clinical endocrinology and metabolism 2001-08, Vol.86 (8), p.3912-3917 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The purpose of this study was to characterize telomerase activity
during the menstrual cycle, focusing on the luteal phase. A total of 84
endometrial biopsy samples were obtained from 72 participants. Daily
urinary LH testing (OvuQuick, Quidel) was used to establish the day of
the LH rise, and participants were randomized to return during the
secretory phase. Twelve women returned on the identical day during the
luteal phase of a subsequent cycle to allow intercycle comparisons of
telomerase activity. Telomerase activity was evaluated using a modified
TRAP-eze (Intergen) detection protocol. At the time of
each endometrial biopsy, serum estrogen and progesterone were measured.
Proliferative phase endometrium showed high telomerase activity. At the
onset of the luteal phase telomerase activity was high, but it
decreased during the early luteal phase, disappeared by the midluteal
phase (6 d after LH surge detected), and then rose to moderate levels
in the late luteal phase beginning on luteal d 10. Serum progesterone
levels were inversely related to telomerase activity.
In conclusion, endometrial telomerase activity is dynamic: high during
the proliferative phase but inhibited during the midsecretory phase of
the menstrual cycle. The timing of expression coincides with the rise
and fall of progesterone levels and the time period of maximal uterine
receptivity for embryo implantation. This supports a relationship
between sex steroid levels and telomerase regulation. |
---|---|
ISSN: | 0021-972X 1945-7197 |
DOI: | 10.1210/jcem.86.8.7729 |