Glucose-Induced Insulin Hypersecretion in Lipid-Infused Healthy Subjects Is Associated with a Decrease in Plasma Norepinephrine Concentration and Urinary Excretion
We investigated the effect of a 48 h triglyceride infusion on the subsequent insulin secretion in response to glucose in healthy men. We measured the variations in plasma concentration and urinary excretion of catecholamines as an indirect estimation of sympathetic tone. For 48 h, 20 volunteers rece...
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Veröffentlicht in: | The journal of clinical endocrinology and metabolism 2001-10, Vol.86 (10), p.4901-4907 |
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Sprache: | eng |
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Zusammenfassung: | We investigated the effect of a 48 h triglyceride infusion on
the subsequent insulin secretion in response to glucose in healthy men.
We measured the variations in plasma concentration and urinary
excretion of catecholamines as an indirect estimation of sympathetic
tone. For 48 h, 20 volunteers received a triglyceride/heparin or a
saline solution, separated by a 1-month interval. At time 48 h,
insulin secretion in response to glucose was investigated by a
single iv glucose injection (0.5 g/kg−1) followed by
an hyperglycemic clamp (10 mg·kg−1·min−1,
during 50 min). The triglyceride infusion resulted in a 3-fold
elevation in plasma free fatty acids and an increase in insulin and
C-peptide plasma concentrations (1.5- and 2.5-fold, respectively,
P < 0.05), compared with saline. At time 48 h
of lipid infusion, plasma norepinephrine (NE) concentration and urinary
excretion levels were lowered compared with saline (plasma NE:
0.65 ± 0.08 vs. 0.42 ± 0.06 ng/ml,
P < 0.05; urinary excretion: 800 ± 70
vs. 620 ± 25 nmol/24 h, P <
0.05). In response to glucose loading, insulin and C-peptide plasma
concentrations were higher in lipid compared with saline infusion
(plasma insulin: 600 ± 98 vs. 310 ± 45
pm, P < 0.05; plasma C-peptide
3.5 ± 0.2 vs. 1.7 ± 0.2 nm,
P < 0.05). In conclusion, in healthy subjects, a
48-h lipid infusion induces basal hyperinsulinemia and exaggerated
insulin secretion in response to glucose which may be partly related to
a decrease in sympathetic tone. |
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ISSN: | 0021-972X 1945-7197 |
DOI: | 10.1210/jcem.86.10.7958 |