Mechanical and physio-biological properties of peptide-coated stent for re-endothelialization

BackgroundThe aim of this study was to characterize the mechanical and physio-biological properties of peptide-coated stent (PCS) compared to commercialized drug-eluting stents (DESs).MethodsWKYMVm (Trp-Lys-Tyr-Met-Val-D-Met), a stimulating peptide for homing endothelial colony-forming cell was specially synthesized and coated to bare metal stent (BMS) by dopamine-derived coordinated bond. Biological effects of PCS were investigated by endothelial cell proliferation assay and pre-clinical animal study. And mechanical properties were examined by various experiment.ResultsThe peptide was well-coated to BMS and was maintained and delivered to 21 and 7days in vitro and in vivo, respectively. Moreover, the proliferation of endothelial cell in PCS group was increased (approximately 36.45.77%) in PCS group at 7day of culture compare to BMS. Although, the radial force of PCS was moderated among study group. The flexibility of PCS was (0.49 +/- 0.082N) was greatest among study group. PCS did not show the outstanding performance in recoil and foreshortening test (3.1 +/- 0.22% and 2.1 +/- 0.06%, respectively), which was the reasonable result under the guide line of FDA (less than 7.0%). The nominal pressure (3.0mm in a diameter) of PCS established by compliance analysis was 9atm. The changing of PCS diameter by expansion was similar to other DESs, which is less than 10atm of pressure for the nominal pressure.Conclusions These results suggest that the PCS is not inferior to commercialized DES. In addition, since the PCS was fabricated as polymer-free process, secondary coating with polymer-based immunosuppressive drugs such as -limus derivatives may possible.