Successful Simultaneous Screening of Sickle Cell Disease, HIV and Tuberculosis in Rural Guinea Bissau, West Africa through Rapid Tests and a Standardized Clinical Questionnaire: An Outreach Program Due to a Public-Private Partnership

Introduction: Previous studies demonstrated that sickle cell disease (SCD), HIV and Tuberculosis (TB) represent high, yet under recognized health issues in Guinea Bissau, West Africa. A public National Reference Center for TB, Hospital Raoul Follereau (HRF) managed by the Italian NGO AHEAD is present in the capital city of Bissau with specialized local health staff capable of managing HIV and TB; the hospital has a SCD Clinic. Access to health services for rural population is limited and in some villages basic health care and routine screening are also seldom available due to lack of resources, specialized staff or diagnostic capacity. Therefore, people are not diagnosed or diagnosed late for SCD, HIV, TB. Nevertheless, rapid tests for both HIV and SCD are available on the market and one for TB is being developed. Simultaneous screening of three big diseases at the point of care (POC) in rural areas would allow saving of time and resources, while identifying people in need of further specialized diagnostic or follow up. Objectives: To evaluate the feasibility of simultaneous screening of HIV, SCD and TB at the POC in a rural area of Guinea Bissau through rapid tests for HIV and SCD and a standardized clinical questionnaire (Figure 1) with clinical examination for TB. To identify patients with HIV, SCD or both and enroll them in appropriate follow up programs; to identify suspects of TB eligible for in depth diagnostic screening. Methods: During the first phase of the project a temporary outreach health post was organized for 10 days in March 2018 in the village of Fanhè, by the NGO AGB already operating in the village. 1) The chiefs of the rural community village informed all the households through collective meetings and home visits regarding the objectives and modalities of the health project after accepting the proposal made by the NGOs and the HRF staff (February-March 2018); 2) the local chiefs organized a schedule of visits according to households; 3) 3 nurses and 2 physicians from the HRF in Bissau, linguistically competent for Creole and local dialects, administered the standardized questionnaire and the physical examination respectively; 3) 2 pediatric residents from Italy aided in clinical examination, a nurse and 2 other physicians in performing the rapid tests for HIV (Determine®) and SCD (Sickle Scan, Biomedomics) after informed consent; patients with Sickle Scan positive results were immediately collected another blood sample on Guthrie card