SENSITIVITY IN ELECTRON MICROSCOPE AUTORADIOGRAPHY I. THE EFFECT OF RADIATION DOSE

Sensitivity in electron microscope autoradiography using Ilford L4 emulsion was shown to be affected by radiation dose (i.e., number of decays in test specimen per unit surface area). The sensitivity tended to be higher with lower doses. This dose dependence was most marked with Microdol X and least...

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Veröffentlicht in:	J. Histochem. Cytochem. 20: No. 6, 425-34(1972) 425-34(1972), 1972-06, Vol.20 (6), p.425-434
Hauptverfasser:	SALPETER, MIRIAM M, SZABO, MARIA
Format:	Artikel
Sprache:	eng
Schlagworte:	Absorption Acrylic Resins Ascorbic Acid AUTORADIOGRAPHY AUTORADIOGRAPHY/sensitivity of electron microscope, effect of radiation dose on CALIBRATION CALIBRATION STANDARDS DEVELOPERS DOSE- RESPONSE RELATIONSHIPS ELECTRON MICROSCOPES ELECTRON MICROSCOPES/calibration procedures for autoradiography Emulsions Gold Methods Microscopy, Electron N46110 -Instrumentation-Radiation Detection Instruments- General Detectors & Monitors NUCLEAR EMULSIONS NUCLEAR EMULSIONS/sensitivity of Ilford L4, effect of developer on dose dependence of Phenols PHOTOGRAPHIC FILMS PHOTOGRAPHY Radiation Dosage SENSITIVITY Sulfites Thiocyanates Tritium
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Zusammenfassung:	Sensitivity in electron microscope autoradiography using Ilford L4 emulsion was shown to be affected by radiation dose (i.e., number of decays in test specimen per unit surface area). The sensitivity tended to be higher with lower doses. This dose dependence was most marked with Microdol X and least with gold latensification-Elon ascorbic acid development. Possible consequences for quantitation in electron microscope autoradiography are discussed.
ISSN:	0022-1554 1551-5044
DOI:	10.1177/20.6.425