EFFECTS OF INFLAMMATION ON SUBPOPULATIONS OF BLOOD LYMPHOCYTES

A method for quantitative cytochemistry has been used to study the surface properties of blood lymphocytes of guinea pigs 24 hr after the production of cell-mediated immunity or acute inflammation. Three subpopulations of small lymphocytes with different surface-binding characteristics for Thorotrast particles have been observed. The average number of particles per micron length of surface membrane, i.e., particle density, has been determined for each class of cells. Only two clusters separated by a distinct gap are apparent in each group of animals. There is a significant decrease in the mean particle density of the major subpopulation of lymphocytes in each group of animals with inflammatory reactions, thereby suggesting a reduction in surface charge. On the other hand, the particle density distribution of the minor population of cells does not differ from normal. The role of surface charge alterations on the circulation, margination and emigration of lymphocytes is discussed.