Ex vivo Evaluation of Tono-Pen and Pneumotonometry in Cat Eyes

Purpose: To evaluate the validity and intraobserver reliability of intraocular pressure (IOP) measurements with both pneumotonometry and the Tono-Pen in a closed ex vivo system in cat eyes. Methods: IOP was increased step by step in 5 enucleated cat eyes, while taking IOP measurements with the Tono-...

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Veröffentlicht in:Ophthalmic research 2006-01, Vol.38 (1), p.13-18
Hauptverfasser: Stoiber, Josef, Fernandez, Viviana, Lamar, Peggy D., Hitzl, Wolfgang, Fantes, Francisco, Parel, Jean-Marie
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Sprache:eng
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Zusammenfassung:Purpose: To evaluate the validity and intraobserver reliability of intraocular pressure (IOP) measurements with both pneumotonometry and the Tono-Pen in a closed ex vivo system in cat eyes. Methods: IOP was increased step by step in 5 enucleated cat eyes, while taking IOP measurements with the Tono-Pen and pneumotonometry. The outcomes were compared to readings of a digital manometer simultaneously measuring the actual pressure in the anterior chamber. Results: Pneumotonometry overestimated IOP below 15 mm Hg and underestimated pressures above 20 mm Hg. Tono-Pen tonometry considerably underestimated IOP over the whole spectrum in all of the eyes tested. The pneumotonometer was identified as the more valid and reliable instrument for cat eyes. Conclusion: Both tonometers are clinically useful tools to assess IOP for glaucoma studies using a cat animal model. However, one has to consider underestimation of IOP in the upper ranges. A correction formula can be used to calculate the actual IOP.
ISSN:0030-3747
1423-0259
DOI:10.1159/000088492