Abstract B48: Integrin α6 S-nitrosylation induces prostate cancer cell migration by loss of adhesion to laminin-1

Prostate cancer is the second most common cancer in American males. Elevated nitric oxide and nitric oxide synthase levels are associated with the progression of prostate tumors. S-nitrosylation, a reversible nitric oxide post-translational modification of cysteines, was investigated here. This report showed that low dose nitric oxide and inducible nitric oxide synthase induced the migration of prostate cancer cells, which was blocked using selective integrin alpha 6 and inducible nitric oxide synthase inhibitors. Furthermore, both nitric oxide donor and inducible nitric oxide synthase targeted integrin alpha 6 for S-nitrosylation on cysteine 86 in prostate cancer cells. Nitric oxide reduced prostate cancer cell adhesion to laminin-1 and integrin alpha 6 binding to laminin beta 1 but theses effects were not seen after mutation of cysteine 86 in integrin alpha 6. S-nitrosylation of integrin alpha 6 cysteine 86 ultimately led to increased migration of prostate cancer cells, which is a potential mechanism of prostate cancer metastasis. Citation Format: Jared Isaac, Ying Wai Lam, Pheruza Tarapore, Shuk-mei Ho. Integrin α6 S-nitrosylation induces prostate cancer cell migration by loss of adhesion to laminin-1 [abstract]. In: Proceedings of the AACR Special Conference on Advances in Prostate Cancer Research; 2012 Feb 6-9; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2012;72(4 Suppl):Abstract nr B48.