Abstract 5036: Induction of highly efficacious anti-tumor activity and modulation of tumor microenvironment: Cell-free off the shelf therapeutic modality

Adoptive transfer of Bispecific antibody Armed activated T cells (BATs) show promising anti-tumor activity in clinical trials in solid tumors. The cytotoxic activity of BATs occur upon engagement with tumor cells via the bispecific antibody bridge which stimulates BATs to release not only the lytic and cytotoxic molecules (perforin/granzyme) but also cytokines, chemokines and other signaling molecules extracellularly. We hypothesized that the release of extracellular soluble factors by this complex interaction of T cells, bispecific antibody, and tumor cells may serve as a potent anti-tumor and immune activating conditioned media (CM). In a 3D tumorsphere model, tumor+BATs-CM (n=10) showed potent cytotoxicity (p