Abstract 5399: Correlation of a microRNA expression profile and the prognosis of penile cancer: A prospective study using microarray data analysis

Introduction and objective: Penile cancer is a rare disease that has high morbidity and mortality rates. While a few biomarkers related to prognosis have been previously described to date none of them was adopted in clinical practice. Our aim was to identify a molecular signature based on miRNA expression levels that could identify those patients with high risk of metastatic penile carcinoma. Methods: We prospectively collected fresh samples of primary tumors from 11 patients with squamous cell penile carcinoma who underwent surgical treatment between July/2015 and June/2017. Five patients had localized disease (non-metastatic group) and 6 had inguinal lymph node metastases (metastatic group). RNA was purified and microarray analysis was performed using miRNA 4.0 Genechip (Affymetrix). We identified differentially expressed miRNAs (DEmiRs) comparing metastatic in relation to non-metastatic groups using TAC Software (Affymetrix), fold change (FC) > 1.5 and p