Abstract 3083: Ameliorative effect of ethanolic extract of Annona muricata against sodium arsenite-induced hepatotoxicty in Wistar rats

Ingestion of arsenic in drinking water causes cancer at multiple tissues and there is no cure. Cancer has become a great monster to human race, as it places a significant emotional and economic burden on families and governments all over the world. Research is therefore directed at chemoprevention using medicinal herbs for the management of arsenicosis. In this study, we evaluated the in vitro antioxidant and protection offered by Annona muricata L. (AM) against sodium arsenite induced hepatotoxicity in rats. Antioxidant and radical scavenging activities of AM were compared to vitamin C and butylated hydroxytoluene (BHT). Proximate and phytochemical analyses were also carried out. Hepatoprotective study was investigated with six groups of rats that received distilled water (Control), 5.0 mg/kg bwt of NaAsO2, 250 mg/kg bwt AM, 500 mg/kg bwt AM, NaAsO2 plus 250 mg/kg AM, NaAsO2 and 500 mg/kg AM. The NaAsO2 was given once on days 7, 14 and 21, while AM was administered orally daily for 21 days. Serum transaminases and alkaline phosphatase activities were determined and liver histopathology carried out. AM contained 2.00% ash, 1.94% crude fat, 25.65% crude fibre, 2.88% protein and 58.62% carbohydrate. Phytochemical analysis indicated the presence of alkaloids, flavonoids and cardiac glycosides. The reducing power and metal chelating ability was in the order Vit C > BHT > AM, while for DPPH scavenging ability AM > Vit C > BHT. The NaAsO2 significantly (p < 0.05) increased the liver function enzymes relative to control. However, AM treatment markedly reduced the marker enzymes and restores the severe vaculation of hepatocytes in the NaAsO2 group to near normal. Our findings suggest that AM may constitute a remedy against arsenic induced hepatic injuries. Citation Format: Adenike M. Adegboyega. Ameliorative effect of ethanolic extract of Annona muricata against sodium arsenite-induced hepatotoxicty in Wistar rats [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 3083.