Abstract 3803: Characterization of fluid biopsy using the HD-SCA platform to re-stratify intermediate-risk organ-confined prostate cancer patients

Recent studies have demonstrated that even with current risk stratification of intermediate-risk organ-confined prostate cancer patients, 30-50% of patients are incorrectly categorized and overtreated. As the second leading cause of cancer in men, 90% of prostate cancer patients are diagnosed at organ-confined stages, where radical prostatectomy (RP) is the “golden standard” of treatment. However, deferring intervention is becoming a more popular option for low-risk patients, with a 15-year progression-free survival of 95%. High-risk patients greatly benefit from RP, with a 5-year progression-free survival of 85%, while the decision to proceed with RP is not so candid for intermediate-risk patients. Using the high-definition single cell assay (HD-SCA) platform, circulating tumor cells (CTCs) from peripheral blood (PB) and disseminating tumor cells (DTCs) from bone marrow aspirate (BMA) will be analyzed for morphological, genomic, and proteomic profiling. We hypothesize that by using morpho-proteo-genomic parameters we may develop predictive markers for a more accurate stratification of intermediate-risk patients, offering a more personalized treatment for each individual. Preliminary data shows 86% (n=43) of PB samples and 5% (n=40) of BMA are positive for Cytokeratin (CK) expressing rare circulating cells, as defined by ≥5 cells/mL. The incidence of CK+ cells in PB did not correlate with PSA, Gleason score, or clinical parameters. However, incidence of CK+ cells in PB was higher in high-risk group, though not statistically significant (p=0.784) when compared to intermediate-risk groups. Additionally, higher levels of CK expression (p