Abstract 749: Characterization of p54nrb/NonO promoter in NSCLC

p54nrb/NonO is a multiple function nuclear protein. It has been implicated in numerous processes within the nucleus including transcriptional regulation, DNA unwinding, nuclear RNA processing, DNA double-strand break repair and tumorigenesis. In vivo, p54nrb usually forms a heterodimer with its partner PSF as a RNA splicing factor, but its roles in tumor progress is unknown. In recent years, p54nrb has been report in bladder cancer and prostate cancer that different expression of p54nrb between normal tissue and tumor tissue. p54nrb is upregulated in bladder cancer and prostate cancer. However, the mechanism about transcriptional regulation of p54nrb is still unknown. In this study, we characterize p54nrb function in non-small cell lung cancer cell (NSCLC) line and the transcriptional regulation of p54nrb promoter. First we find p54nrb transcription start site and use PCR technology to clone -1671/+1261 (2952 bp) p54nrb promoter. Then we analyzed p54nrb promoter region by deletion of p54nrb promoter 5’ end and luciferase report assay. The results of luciferase assay demonstrated that there is an important regulation region in p54nrb promoter +311/+414 fragment. And deletion of p54nrb promoter +109/+203 region can cause a part of luciferase activity decreasing. Furthermore, we used programe to predict what kind of transcription factor binding site on these two p54nrb promoter region. And we found three CCAAT box and AP-1, AP2α, Sp1, PEA3, NF-1, YY1 binding site. To investigate which transcription factors involve in transcriptional regulation of p54nrb. We observed the change of p54nrb after overexpressed AP-1, AP2α and Sp1 in CL1-0, CL1-5 and A549 cell line, and the protein level and mRNA level of p54nrb didn't change after overexpressed AP-1, AP2α and Sp1. Citation Format: Sheng-Yi Lin, Yi-Hua Lai, Hsuan-Yu Chen, Huei-Wen Chen, Sung-Liang Yu, Tse-Ming Hong, Gee-Chen Chang, Jeremy J.W. Chen. Characterization of p54nrb/NonO promoter in NSCLC. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 749. doi:10.1158/1538-7445.AM2013-749 Note: This abstract was not presented at the AACR Annual Meeting 2013 because the presenter was unable to attend.