Abstract 200: Identification of proteins involved in c-Myc induced mammary epithelia apoptosis in 3D culture system

Identification of proteins involved in c-Myc induced mammary epithelia apoptosis in 3D culture system We have previously discovered that depletion of a cell polarity gene Scribble in mammary epithelial cells disrupted cell polarity, apoptosis and morphogenesis in three dimensional cell culture as well as inducing dysplasia that progressed into tumor in mice. Loss of Scribble also inhibited c-Myc induced apoptosis to cause cell transformation in vitro and tumor formation in vivo. Scribble mutant with deficiency in membrane localization resulted in similar phenotypes. These results suggest that Scribble is a tumor suppressor in mammary gland epithelial cells and its functions depends on correct cellular localization. Furthermore, Scribble is required to inhibit Myc-induced tumorigenesis by blocking Myc-induced apoptosis. I am interested in identifying proteins that are involved in Scribble dependent apoptosis as well as discovering other polarity proteins that cooperate with c-Myc for mammary tumor progression. To address the two questions, I use MCF10A three dimensional culture system and RNAi technology to screen proteins participating in apoptosis during c-Myc induced mammary epithelial cell transformation. We will report a screen to identify proteins that are involved in c-Myc induced mammary epithelial cell apoptosis in 3D culture, using a lentiviral shRNA library. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 200. doi:10.1158/1538-7445.AM2011-200