Abstract 415: Identification of colon cancer recurrence related CNVs using array-CGH

Colon cancer is one of the leading causes for cancer-related death in the Western world. The most important factor in colon cancer-related death is recurrence. Recently, many studies have reported that copy number variation (CNV) is related to genetic diseases such as cancer and Alzheimer's disease. In this study, we selected the different aberration CNVs from non-tumor colon tissues by comparing recurred and non-recurred colon cancer patients using 44K oligonucleotide microarray. We selected 1359 different aberration probes, which contained cancer-, neurological disease- and cell signaling-related pathway genes between 22 non-recurred and 19 recurred patients. In 1359 probes, Ingenuity Pathway Analysis software showed that 89 genes composed of cell to cell signaling, cellular movement, and nervous system developmental pathway. In these 89 genes, LIMS2 was known as ILK-related molecular function of cancer cell migration and invasion. ILK-related pathway had important role in cancer cell migration and invasion through function of secretory protein such as MMP9, MMP11. LIMS2 showed copy number amplification and up-regulation of mRNA expression in non-recurrence patient group. In vitro experiment, protein and mRNA of LIMS2 were down-expressed in 5 colon cancer cell lines compared to 3 normal epithelial colon cell lines. In LIMS2 siRNA treated HCT-116 colon cancer cell, protein level of LIMS2 was decreased about 90%, while MMP9, MMP11 protein expression increased. Also, migration and invasion rate of HCT-116 cell increased in LIMS2 siRNA treated group and LIMS2 siRNA treated condition media compared to the control group using wound-healing and trans-well assay. According to array-CGH and in vitro experiment, we found that LIMS2 could play an important role in colon cancer metastasis through paracrine effect. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 415.