A Study on Gentiana dahurica Fisch Ethanol Extract Alleviating Alcoholic Liver Disease in Mice: A Metabolomic Analysis of the Liver

A Study on Gentiana dahurica Fisch Ethanol Extract Alleviating Alcoholic Liver Disease in Mice: A Metabolomic Analysis of the Liver

We clarified the hepatoprotective effect of Gentiana dahurica Fisch ethanol extract (GDEE) in our previous study, and we further revealed the mechanism with the help of metabolomics technology in this study. The livers from Control group, Alcohol group, and Alcohol + GDEE group were analyzed by meta...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Evidence-based complementary and alternative medicine 2021-06, Vol.2021, p.1-7, Article 5569538
Hauptverfasser: Cao, Houkang, Guo, Yanxiu, Jin, Ling
Format: Artikel
Sprache:eng
Schlagworte:
Alcohol
Amino acids
Correlation analysis
Data analysis
Ethanol
Gentiana
Glucose metabolism
High-performance liquid chromatography
Integrative & Complementary Medicine
Life Sciences & Biomedicine
Lipid metabolism
Liver diseases
Mass spectroscopy
Metabolism
Metabolites
Metabolomics
Principal components analysis
Quality control
Science & Technology
Software
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 7
container_issue
container_start_page 1
container_title Evidence-based complementary and alternative medicine
container_volume 2021
creator Cao, Houkang
Guo, Yanxiu
Jin, Ling
description We clarified the hepatoprotective effect of Gentiana dahurica Fisch ethanol extract (GDEE) in our previous study, and we further revealed the mechanism with the help of metabolomics technology in this study. The livers from Control group, Alcohol group, and Alcohol + GDEE group were analyzed by metabolomics. The metabolites in the liver were separated by ultra-high-performance liquid chromatography (UHPLC) and were tentatively identified using mass spectrometry (MS)/MS analysis. Differential metabolites were defined with VIP > 1 and P
doi_str_mv 10.1155/2021/5569538
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1155_2021_5569538</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2550176996</sourcerecordid><originalsourceid>FETCH-LOGICAL-c425t-1fcdb39dd2ad1226c442f511cc23c780226739dcde90d538e2c35d7707b24c033</originalsourceid><addsrcrecordid>eNqNkcFuEzEQhi0EoiVw4wEscUGCUNu7Xu9yQFqFtCCl4gBI3CzHnu26cuzW9qbkzIvjkigIDoiTR55vZv6ZH6HnlLyhlPMzRhg947zpeNU-QKdU1HRes7Z9eIzFtxP0JKVrQlgnhHiMTqqacdG09Sn60ePPeTI7HDy-AJ-t8gobNU7RaoXPbdIjXuZR-eDw8nuOSmfcOwdbq7L1VyXWYQzOaryyW4j4vU2gEmDr8aXV8Bb3-BKyWgcXNgXqvXK7ZBMOA84j7IueokeDcgmeHd4Z-nq-_LL4MF99uvi46FdzXeTmOR20WVedMUwZylij65oNnFKtWaVFS8qXKGltoCOmHAOYrrgRgog1qzWpqhl6t-97M603YHRZNyonb6LdqLiTQVn5Z8bbUV6FrWxZQyrKSoOXhwYx3E6QstyUA4FzykOYkmScUy7qruiYoRd_oddhimX7XxShoum6plCv95SOIaUIw1EMJfLeXXnvrjy4W_BXe_wO1mFI2oLXcCwhhDSibghnJSK00O3_0wubi6HBL8Lk8-9Bo_VG3dl_y_oJKuvDew</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2550176996</pqid></control><display><type>article</type><title>A Study on Gentiana dahurica Fisch Ethanol Extract Alleviating Alcoholic Liver Disease in Mice: A Metabolomic Analysis of the Liver</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>Wiley-Blackwell Open Access Titles</source><source>Web of Science - Science Citation Index Expanded - 2021&lt;img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /&gt;</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Cao, Houkang ; Guo, Yanxiu ; Jin, Ling</creator><contributor>Hu, Weicheng ; Weicheng Hu</contributor><creatorcontrib>Cao, Houkang ; Guo, Yanxiu ; Jin, Ling ; Hu, Weicheng ; Weicheng Hu</creatorcontrib><description>We clarified the hepatoprotective effect of Gentiana dahurica Fisch ethanol extract (GDEE) in our previous study, and we further revealed the mechanism with the help of metabolomics technology in this study. The livers from Control group, Alcohol group, and Alcohol + GDEE group were analyzed by metabolomics. The metabolites in the liver were separated by ultra-high-performance liquid chromatography (UHPLC) and were tentatively identified using mass spectrometry (MS)/MS analysis. Differential metabolites were defined with VIP &gt; 1 and P&lt;0.05. Principal component analysis (PCA) and orthogonal partial least square discriminant analysis (OPLS-DA) were applied to analyze differences among these groups. The results showed that the groups could be clearly distinguished by PCA and OPLS-DA analysis. Alcohol and GDEE could change the overall profile of liver metabolites. Alterations in liver tissues of ALD mice induced by alcohol were mainly involved in the dipeptides, purine and pyrimidine metabolism and glucose and lipid metabolism, which could be partly affected by GDEE. This study revealed that the mechanism of GDEE in alleviating ALD had the characteristics of multitarget and multipathway.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2021/5569538</identifier><identifier>PMID: 34257684</identifier><language>eng</language><publisher>LONDON: Hindawi</publisher><subject>Alcohol ; Amino acids ; Correlation analysis ; Data analysis ; Ethanol ; Gentiana ; Glucose metabolism ; High-performance liquid chromatography ; Integrative &amp; Complementary Medicine ; Life Sciences &amp; Biomedicine ; Lipid metabolism ; Liver diseases ; Mass spectroscopy ; Metabolism ; Metabolites ; Metabolomics ; Principal components analysis ; Quality control ; Science &amp; Technology ; Software</subject><ispartof>Evidence-based complementary and alternative medicine, 2021-06, Vol.2021, p.1-7, Article 5569538</ispartof><rights>Copyright © 2021 Houkang Cao et al.</rights><rights>Copyright © 2021 Houkang Cao et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2021 Houkang Cao et al. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>2</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000674605200001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c425t-1fcdb39dd2ad1226c442f511cc23c780226739dcde90d538e2c35d7707b24c033</citedby><cites>FETCH-LOGICAL-c425t-1fcdb39dd2ad1226c442f511cc23c780226739dcde90d538e2c35d7707b24c033</cites><orcidid>0000-0001-5496-6343 ; 0000-0001-6124-4859</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260312/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260312/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,39263,53796,53798</link.rule.ids></links><search><contributor>Hu, Weicheng</contributor><contributor>Weicheng Hu</contributor><creatorcontrib>Cao, Houkang</creatorcontrib><creatorcontrib>Guo, Yanxiu</creatorcontrib><creatorcontrib>Jin, Ling</creatorcontrib><title>A Study on Gentiana dahurica Fisch Ethanol Extract Alleviating Alcoholic Liver Disease in Mice: A Metabolomic Analysis of the Liver</title><title>Evidence-based complementary and alternative medicine</title><addtitle>EVID-BASED COMPL ALT</addtitle><description>We clarified the hepatoprotective effect of Gentiana dahurica Fisch ethanol extract (GDEE) in our previous study, and we further revealed the mechanism with the help of metabolomics technology in this study. The livers from Control group, Alcohol group, and Alcohol + GDEE group were analyzed by metabolomics. The metabolites in the liver were separated by ultra-high-performance liquid chromatography (UHPLC) and were tentatively identified using mass spectrometry (MS)/MS analysis. Differential metabolites were defined with VIP &gt; 1 and P&lt;0.05. Principal component analysis (PCA) and orthogonal partial least square discriminant analysis (OPLS-DA) were applied to analyze differences among these groups. The results showed that the groups could be clearly distinguished by PCA and OPLS-DA analysis. Alcohol and GDEE could change the overall profile of liver metabolites. Alterations in liver tissues of ALD mice induced by alcohol were mainly involved in the dipeptides, purine and pyrimidine metabolism and glucose and lipid metabolism, which could be partly affected by GDEE. This study revealed that the mechanism of GDEE in alleviating ALD had the characteristics of multitarget and multipathway.</description><subject>Alcohol</subject><subject>Amino acids</subject><subject>Correlation analysis</subject><subject>Data analysis</subject><subject>Ethanol</subject><subject>Gentiana</subject><subject>Glucose metabolism</subject><subject>High-performance liquid chromatography</subject><subject>Integrative &amp; Complementary Medicine</subject><subject>Life Sciences &amp; Biomedicine</subject><subject>Lipid metabolism</subject><subject>Liver diseases</subject><subject>Mass spectroscopy</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Metabolomics</subject><subject>Principal components analysis</subject><subject>Quality control</subject><subject>Science &amp; Technology</subject><subject>Software</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>HGBXW</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkcFuEzEQhi0EoiVw4wEscUGCUNu7Xu9yQFqFtCCl4gBI3CzHnu26cuzW9qbkzIvjkigIDoiTR55vZv6ZH6HnlLyhlPMzRhg947zpeNU-QKdU1HRes7Z9eIzFtxP0JKVrQlgnhHiMTqqacdG09Sn60ePPeTI7HDy-AJ-t8gobNU7RaoXPbdIjXuZR-eDw8nuOSmfcOwdbq7L1VyXWYQzOaryyW4j4vU2gEmDr8aXV8Bb3-BKyWgcXNgXqvXK7ZBMOA84j7IueokeDcgmeHd4Z-nq-_LL4MF99uvi46FdzXeTmOR20WVedMUwZylij65oNnFKtWaVFS8qXKGltoCOmHAOYrrgRgog1qzWpqhl6t-97M603YHRZNyonb6LdqLiTQVn5Z8bbUV6FrWxZQyrKSoOXhwYx3E6QstyUA4FzykOYkmScUy7qruiYoRd_oddhimX7XxShoum6plCv95SOIaUIw1EMJfLeXXnvrjy4W_BXe_wO1mFI2oLXcCwhhDSibghnJSK00O3_0wubi6HBL8Lk8-9Bo_VG3dl_y_oJKuvDew</recordid><startdate>20210629</startdate><enddate>20210629</enddate><creator>Cao, Houkang</creator><creator>Guo, Yanxiu</creator><creator>Jin, Ling</creator><general>Hindawi</general><general>Hindawi Publishing Group</general><general>Hindawi Limited</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5496-6343</orcidid><orcidid>https://orcid.org/0000-0001-6124-4859</orcidid></search><sort><creationdate>20210629</creationdate><title>A Study on Gentiana dahurica Fisch Ethanol Extract Alleviating Alcoholic Liver Disease in Mice: A Metabolomic Analysis of the Liver</title><author>Cao, Houkang ; Guo, Yanxiu ; Jin, Ling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-1fcdb39dd2ad1226c442f511cc23c780226739dcde90d538e2c35d7707b24c033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Alcohol</topic><topic>Amino acids</topic><topic>Correlation analysis</topic><topic>Data analysis</topic><topic>Ethanol</topic><topic>Gentiana</topic><topic>Glucose metabolism</topic><topic>High-performance liquid chromatography</topic><topic>Integrative &amp; Complementary Medicine</topic><topic>Life Sciences &amp; Biomedicine</topic><topic>Lipid metabolism</topic><topic>Liver diseases</topic><topic>Mass spectroscopy</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Metabolomics</topic><topic>Principal components analysis</topic><topic>Quality control</topic><topic>Science &amp; Technology</topic><topic>Software</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cao, Houkang</creatorcontrib><creatorcontrib>Guo, Yanxiu</creatorcontrib><creatorcontrib>Jin, Ling</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Psychology Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Evidence-based complementary and alternative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cao, Houkang</au><au>Guo, Yanxiu</au><au>Jin, Ling</au><au>Hu, Weicheng</au><au>Weicheng Hu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Study on Gentiana dahurica Fisch Ethanol Extract Alleviating Alcoholic Liver Disease in Mice: A Metabolomic Analysis of the Liver</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><stitle>EVID-BASED COMPL ALT</stitle><date>2021-06-29</date><risdate>2021</risdate><volume>2021</volume><spage>1</spage><epage>7</epage><pages>1-7</pages><artnum>5569538</artnum><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>We clarified the hepatoprotective effect of Gentiana dahurica Fisch ethanol extract (GDEE) in our previous study, and we further revealed the mechanism with the help of metabolomics technology in this study. The livers from Control group, Alcohol group, and Alcohol + GDEE group were analyzed by metabolomics. The metabolites in the liver were separated by ultra-high-performance liquid chromatography (UHPLC) and were tentatively identified using mass spectrometry (MS)/MS analysis. Differential metabolites were defined with VIP &gt; 1 and P&lt;0.05. Principal component analysis (PCA) and orthogonal partial least square discriminant analysis (OPLS-DA) were applied to analyze differences among these groups. The results showed that the groups could be clearly distinguished by PCA and OPLS-DA analysis. Alcohol and GDEE could change the overall profile of liver metabolites. Alterations in liver tissues of ALD mice induced by alcohol were mainly involved in the dipeptides, purine and pyrimidine metabolism and glucose and lipid metabolism, which could be partly affected by GDEE. This study revealed that the mechanism of GDEE in alleviating ALD had the characteristics of multitarget and multipathway.</abstract><cop>LONDON</cop><pub>Hindawi</pub><pmid>34257684</pmid><doi>10.1155/2021/5569538</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-5496-6343</orcidid><orcidid>https://orcid.org/0000-0001-6124-4859</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1741-427X
ispartof Evidence-based complementary and alternative medicine, 2021-06, Vol.2021, p.1-7, Article 5569538
issn 1741-427X
1741-4288
language eng
recordid cdi_crossref_primary_10_1155_2021_5569538
source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Wiley-Blackwell Open Access Titles; Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; PubMed Central; Alma/SFX Local Collection
subjects Alcohol
Amino acids
Correlation analysis
Data analysis
Ethanol
Gentiana
Glucose metabolism
High-performance liquid chromatography
Integrative & Complementary Medicine
Life Sciences & Biomedicine
Lipid metabolism
Liver diseases
Mass spectroscopy
Metabolism
Metabolites
Metabolomics
Principal components analysis
Quality control
Science & Technology
Software
title A Study on Gentiana dahurica Fisch Ethanol Extract Alleviating Alcoholic Liver Disease in Mice: A Metabolomic Analysis of the Liver
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-15T05%3A36%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20Study%20on%20Gentiana%20dahurica%20Fisch%20Ethanol%20Extract%20Alleviating%20Alcoholic%20Liver%20Disease%20in%20Mice:%20A%20Metabolomic%20Analysis%20of%20the%20Liver&rft.jtitle=Evidence-based%20complementary%20and%20alternative%20medicine&rft.au=Cao,%20Houkang&rft.date=2021-06-29&rft.volume=2021&rft.spage=1&rft.epage=7&rft.pages=1-7&rft.artnum=5569538&rft.issn=1741-427X&rft.eissn=1741-4288&rft_id=info:doi/10.1155/2021/5569538&rft_dat=%3Cproquest_cross%3E2550176996%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2550176996&rft_id=info:pmid/34257684&rfr_iscdi=true